Dissociation constants for carbonic anhydrase-sulfonamide binding by high-performance liquid chromatography

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Dissociation constants for carbonic anhydrase--sulfonamide binding by high-performance liquid chromatography.

Carbonic anhydrase-azosulfonamide dissociation constants (Kd) were determined by gel filtration with high-performance liquid chromatography. By measuring the area of the elution peak at two wavelengths, Kd values were derived without having to measure a shallow trough. The procedure proved to be fast and reliable and has a general application. The dissociation constants were measured for 7-acet...

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ژورنال

عنوان ژورنال: Analytical Biochemistry

سال: 1984

ISSN: 0003-2697

DOI: 10.1016/0003-2697(84)90089-7